Correlation Between Sperm Membrane Destabilization by Heparin and Aniline Blue Staining as Membrane Integrity Index
نویسندگان
چکیده
منابع مشابه
Evaluation of Protamine Level in Human Sperm Samples Using Chromomycin A3 and Aniline Blue Staining
Background: Current microscopic experimental methods cannot diagnose DNA damages present in spermatozoa .Therefore, some methods are needed to address the abnormality of the genetic material status on the sperm samples. As reported by many investigators aniline blue staining technique has been used for identifying sperm chromatin condensation. Also, chromomycin A3 is used for evaluation of the ...
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The aim of this study was to determine whether flow cytometric evaluation of combined merocyanine 540 and Yo-Pro 1 (M540-YP) staining would identify viable dog sperm that had undergone membrane stabilization known to be associated with capacitation in other species, and whether such destabilization is detected earlier than when using the tyrosine phosphorylation and ethidium homodimer (TP-EH) s...
متن کاملEffect of PolyVinyl Pyrrolidone on Sperm Membrane Integrity and Chromatin Status
Background The aim of present study is to evaluate the effect of PolyVinyl Pyrrolidone (PVP) routinly used during ICSI procedure on sperm membrane integrity, and sperm chromatin status. MaterialsAndMethods This study was carried out on 21 semen samples from the infertile men referring to Isfahan Fertility and Infertility Center. The processed semen samples were divided into two portions. One po...
متن کاملThe utility of sperm DNA damage assay using toluidine blue and aniline blue staining in routine semen analysis
OBJECTIVE The aim of the present study was to examine the relationship among male age, strict morphology, and sperm chromatin structure and condensation. METHODS Sperm samples from a total of 100 men underwent semen analysis, and sperm chromatin structure and condensation were assessed with toluidine blue (TB) and aniline blue (AB) tests. RESULTS Prevalence of strict morphology of less than...
متن کاملthe effect of varicocelectomy on sperm parameters, membrane integrity and sperm chromatin condensation
introduction: varicocele is considered as a common cause of male infertility. the aim of this study was to evaluate the effects of varicocelectomy on sperm parameters, sperm membrane integrity and sperm nuclear maturity. materials and methods: in this study along with routine semen analysis, hypo-osmotic swelling test (host), and aniline blue staining for assessment of as 45 and 90 days after i...
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ژورنال
عنوان ژورنال: Archives of Andrology
سال: 1998
ISSN: 0148-5016
DOI: 10.3109/01485019808987937